Current smoking with or without chronic bronchitis is independently associated with goblet cell hyperplasia in healthy smokers and COPD subjects.

TitleCurrent smoking with or without chronic bronchitis is independently associated with goblet cell hyperplasia in healthy smokers and COPD subjects.
Publication TypePublication
Year2020
AuthorsKim V, Jeong S, Zhao H, Kesimer M, Boucher RC, J Wells M, Christenson SA, Han MK, Dransfield M, Paine R, Cooper CB, Barjaktarevic I, Bowler R, Curtis JL, Kaner RJ, O'Beirne SL, O'Neal WK, Rennard SI, Martinez FJ, Woodruff PG
JournalSci Rep
Volume10
Issue1
Pagination20133
Date Published2020 11 18
ISSN2045-2322
KeywordsAged, Bronchitis, Chronic, Female, Goblet Cells, Humans, Hyperplasia, Logistic Models, Male, Middle Aged, Prospective Studies, Pulmonary Disease, Chronic Obstructive, Smokers, smoking
Abstract

COPD, chronic bronchitis (CB) and active smoking have all been associated with goblet cell hyperplasia (GCH) in small studies. Active smoking is strongly associated with CB, but there is a disconnect between CB clinical symptoms and pathology. Chronic cough and sputum production poorly correlate with the presence of GCH or COPD. We hypothesized that the primary determinant of GCH in ever smokers with or without airflow obstruction is active smoking. Goblet Cell Density (GCD) was measured in 71 current or former smokers [32 subjects without COPD and 39 COPD subjects]. Endobronchial mucosal biopsies were stained with Periodic Acid Schiff-Alcian Blue, and GCD was measured as number of goblet cells/mm basement membrane. GCD was divided into tertiles based on log transformed values. LogGCD was greater in current smokers compared to former smokers. Those with classically defined CB or SGRQ defined CB had a greater log GCD compared to those without CB. Current smoking was independently associated with tertile 3 (high logGCD) whereas CB was not in multivariable regression when adjusting for lung function and demographics. These results suggest that GCH is induced by active smoke exposure and does not necessarily correlate with the clinical symptoms of CB.

DOI10.1038/s41598-020-77229-1
Alternate JournalSci Rep
PubMed ID33208859
PubMed Central IDPMC7674445
Grant ListK23 HL123778 / HL / NHLBI NIH HHS / United States
K24 HL137013 / HL / NHLBI NIH HHS / United States
U01 HL137880 / HL / NHLBI NIH HHS / United States
U24 HL141762 / HL / NHLBI NIH HHS / United States
MS#: 
MS085
Manuscript Full Title: 
Current smoking with or without chronic bronchitis is independently associated with goblet cell hyperplasia in healthy smokers and COPD subjects.
Manuscript Lead/Corresponding Author Affiliation: 
Clinical Center: Pennsylvania (Temple University)
ECI: 
Manuscript Status: 
Published and Public