Comparison of serum, EDTA plasma and P100 plasma for luminex-based biomarker multiplex assays in patients with chronic obstructive pulmonary disease in the SPIROMICS study.

TitleComparison of serum, EDTA plasma and P100 plasma for luminex-based biomarker multiplex assays in patients with chronic obstructive pulmonary disease in the SPIROMICS study.
Publication TypePublication
AuthorsO'Neal WK, Anderson W, Basta PV, Carretta EE, Doerschuk CM, R Barr G, Bleecker ER, Christenson SA, Curtis JL, Han MK, Hansel NN, Kanner RE, Kleerup EC, Martinez FJ, Miller BE, Peters SP, Rennard SI, Scholand MBeth, Tal-Singer R, Woodruff PG, Couper DJ, Davis SM
Corporate AuthorsSPIROMICS Investigators
JournalJ Transl Med
Date Published2014 Jan 08
Keywordsbiomarkers, Diagnostic Techniques, Respiratory System, Edetic Acid, Female, Humans, Male, Middle Aged, Plasma, Protease Inhibitors, Pulmonary Disease, Chronic Obstructive, Reproducibility of Results, serum

BACKGROUND: As a part of the longitudinal Chronic Obstructive Pulmonary Disease (COPD) study, Subpopulations and Intermediate Outcome Measures in COPD study (SPIROMICS), blood samples are being collected from 3200 subjects with the goal of identifying blood biomarkers for sub-phenotyping patients and predicting disease progression. To determine the most reliable sample type for measuring specific blood analytes in the cohort, a pilot study was performed from a subset of 24 subjects comparing serum, Ethylenediaminetetraacetic acid (EDTA) plasma, and EDTA plasma with proteinase inhibitors (P100).METHODS: 105 analytes, chosen for potential relevance to COPD, arranged in 12 multiplex and one simplex platform (Myriad-RBM) were evaluated in duplicate from the three sample types from 24 subjects. The reliability coefficient and the coefficient of variation (CV) were calculated. The performance of each analyte and mean analyte levels were evaluated across sample types.RESULTS: 20% of analytes were not consistently detectable in any sample type. Higher reliability and/or smaller CV were determined for 12 analytes in EDTA plasma compared to serum, and for 11 analytes in serum compared to EDTA plasma. While reliability measures were similar for EDTA plasma and P100 plasma for a majority of analytes, CV was modestly increased in P100 plasma for eight analytes. Each analyte within a multiplex produced independent measurement characteristics, complicating selection of sample type for individual multiplexes.CONCLUSIONS: There were notable detectability and measurability differences between serum and plasma. Multiplexing may not be ideal if large reliability differences exist across analytes measured within the multiplex, especially if values differ based on sample type. For some analytes, the large CV should be considered during experimental design, and the use of duplicate and/or triplicate samples may be necessary. These results should prove useful for studies evaluating selection of samples for evaluation of potential blood biomarkers.

Alternate JournalJ Transl Med
PubMed ID24397870
PubMed Central IDPMC3928911
Grant ListHHSN268200900017C / / PHS HHS / United States
UL1TR000124 / TR / NCATS NIH HHS / United States
P30 ES010126 / ES / NIEHS NIH HHS / United States
HHSN268200900015C / / PHS HHS / United States
HHSN268200900020C / / PHS HHS / United States
HHSN268200900018C / / PHS HHS / United States
UL1 TR000124 / TR / NCATS NIH HHS / United States
HHSN268200900014C / / PHS HHS / United States
HHSN2682009000019C / / PHS HHS / United States
HHSN268200900013C / / PHS HHS / United States
HHSN268200900016C / / PHS HHS / United States
Manuscript Lead/Corresponding Author Affiliation: 
Genomics and Informatics Center (University of North Carolina at Chapel Hill)
Manuscript Status: